Collecting Fractions Automatically (Autopurification)

Chromeleon supports automatic fraction collection. This is also referred to as Autopurification. Autopurification includes three steps:

• Pre-analysis of the preparative samples

• Preparative purification of the remaining samples, based on the pre-analysis results

• Post-analysis of the collected fractions for purity and yield evaluation

In order to perform autopurification, make sure that

1. An Extended Fraction Collection license is installed.

2. A Dionex Sample and Fraction Manager is installed. (For installation details, refer to Installing Dionex Devices  HPLC Sample and Fraction Manager (SFM) in the Administrator Help section.)

For general information about fraction collection, refer to  Collecting Fractions.

For more information about autopurification, refer to:

 Setting up Autopurification

 Creating PGM Files for Autopurification Samples

 Using Triggers in Autopurification Programs

 Performing Autopurification

 Autopurification Samples in the Sample List

 Autopurification Samples in the Chromatogram

 Autopurification Samples in the Tray Views